Printing Arrays

 

The preferred method for printing nucleic acid microarrays uses the OmniGrid by GeneMachines. This robot uses up to 48 quill-tip pins to print up to 100 slides. The pins take up about 0.5 µl when loading and print about 1nl onto each slide. Between samples, the pins can be washed with a sonicator or a water bath and dried by vacuum. The robot takes about one hour to set up and can print a 384-well plate in about 20 minutes when using 32 pins.

 

 

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Microarrays

Homemade Microarrays

Commercial: Affymetrix

Commerical: Agilent

Alphabetical List of Microarray Protocols

Real-Time PCR Techniques

Choosing a Reaction Chemistry

Opticon Protocol (MS Word)

Stratagene MX3000p Protocol (MS Word)

Designing Real Time PCR Experiments (MS Word)

Flow Cytometry

Instrumentation Overview

Policies and Fees

Links

HPLC and Mass Spec Techniques

Capillary-Based

Illumina