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Illumina Sequencing
Researchers can choose either single or paired-end reads. Multiple samples can be indexed (barcoded) and be combined in order to make the most of the system's output. Standard flow cells contain 8 lanes which can each generate up to 200 million clusters. All the lanes on a flow cell must be used at the same time, but they may be shared by different groups. If you are interested in sharing a flow cell, please talk to us and we'll try to help match you with someone. This type of flow cell is most effective for running single end 50bp reads. Rapid flow cells contain 2 lanes which can either recieve the same sample or can be split between two samples. Each of these lanes can generate up to 150 million clusters. These flow cells run much more quickly than standard flow cells. We support single and paired-end reads of 50, 100 or 150 bp on rapid runs. MiSeq flow cells contain a single lane that can generate up to 12 million clusters. These flow cells run quickly and allow reads up to 250 bp long, making them especially useful for hard-to-align projects. We also offer library QC on the MiSeq allowing you to run a library quickly and cheaply at low throughput before comitting to a more expensive high throughput sequencing project. We will combine up to four researcher's samples on one MiSeq lane as long as they are differently indexed. Before beginning a project, please contact Christian Daly (cdaly@cgr.harvard.edu) to discuss your experiment. See the link below for sample preparation information. When you are ready to submit samples, please use our online sample submission system. New users will need to register in the system before logging in. For sample and submission questions, please email illumina_submission-list@lists.fas.harvard.edu. If you have problems with the sample submission system, please email rchelp@fas.harvard.edu. For pricing, please see our price list.
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